Plasmid_Backbone

Part:BBa_K4404007:Design

Designed by: Julia Fricke   Group: iGEM22_Goettingen   (2022-10-09)

pMTL83151


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 375
    Illegal EcoRI site found at 3850
    Illegal XbaI site found at 3877
    Illegal PstI site found at 3740
    Illegal PstI site found at 3916
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 375
    Illegal EcoRI site found at 3850
    Illegal NheI site found at 4095
    Illegal PstI site found at 3740
    Illegal PstI site found at 3916
    Illegal NotI site found at 3819
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 375
    Illegal EcoRI site found at 3850
    Illegal BglII site found at 3904
    Illegal BamHI site found at 3871
    Illegal XhoI site found at 3908
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 375
    Illegal EcoRI site found at 3850
    Illegal XbaI site found at 3877
    Illegal PstI site found at 3740
    Illegal PstI site found at 3916
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 375
    Illegal EcoRI site found at 3850
    Illegal XbaI site found at 3877
    Illegal PstI site found at 3740
    Illegal PstI site found at 3916
    Illegal NgoMIV site found at 1289
    Illegal NgoMIV site found at 3196
    Illegal NgoMIV site found at 3206
    Illegal NgoMIV site found at 3332
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 2948
    Illegal SapI.rc site found at 3303
    Illegal SapI.rc site found at 3674


Design Notes

It is important to use inserts with specific restriction enzymes in order to secure a robust implementation into a vector system.


Source

ClosTron modified by Prof. Peter Dürre (department of microbiology and biotechnology, university of Ulm).

References

Heap, J. T.; Pennington, O. J.; Cartman, S. T.; and Minton, N. P. (2009). A modular system for Clostridium shuttle plasmids. J. Microbiol. Methods 78, 79–85. doi:10.1016/j.mimet.2009.05.004